• 介紹： N-(2-羥乙基)哌嗪-N’-(2-乙磺酸)，是可滿足于所有生物學研究的最佳緩沖液之一。絕大部分生理PHS下HEPES是一種兩性離子有機化學緩沖劑，屬于Good’s緩沖試劑之一，pH 6.0-8.5范圍內的緩沖能力最強。HEPES應用非常廣泛，包括：1）用作細胞培養(yǎng)的緩沖體系，常用工作濃度為10-25 mM；與無機碳酸氫鹽緩沖系統(tǒng)相比，在組織或者器官培養(yǎng)中表現(xiàn)出更好的pH控制能力。不像碳酸氫鹽，HEPES不為細胞供給營養(yǎng)。只有當需要長時間在CO2培養(yǎng)箱內處理細胞，才會加入培養(yǎng)基內以增強緩沖能力。2）用作兩性電解質溶液；HEPES能溶解兩性電解質，制備＜1 pH單位寬度的pH梯度以用于等電聚焦電泳實驗；3）用作電鏡觀察蛋白沉積技術合適的緩沖體系，因HEPES不會對金屬基板造成負面影響；4）可用作定量和選擇性測定抗原-抗體反應的緩沖試劑。

• 熔點： 234-238℃

• 外觀： 白色結晶粉末

• 溶解性： 溶于水(2.25mol/L,0℃)

• 儲存條件： RT

• 用途： 本品僅供科研，不得用于其它用途。

• 注意：部分產品我司僅能提供部分信息，我司不保證所提供信息的權威性，僅供客戶參考交流研究之用。

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輸入產品批號：

本計算器可幫助您計算出特定溶液中溶質的質量、溶液濃度和體積之間的關系，公式為：

質量 (mg) = 濃度 (mM) x 體積 (mL) x 分子摩爾量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *